Quebracho (Qu) and chestnut (Ch) are natural sources of tannins and they are currently used 5-Piece Dining Set in animal nutrition as feed ingredients.However, to date the bio-accessibility, antimicrobial, antioxidant, and intestinal epithelial cell stimulatory doses of Qu and Ch have not been determined.Our study investigates the antioxidant and E.
coli F4+ and F18+ growth inhibitory activity of Qu, Ch, and their combinations after solubilization in water (to evaluate the already bio-accessible molecules) and after simulated gastro-intestinal digestion in vitro.The effect of an in vitro digested Ch and Qu combination was also tested on intestinal epithelial IPEC-J2 cells experimentally stressed with hydrogen peroxide (H2O2) and Dextran Sodium Sulfate (DSS).The results showed that undigested Qu and Ch alone, and in combination, exerted a valuable antioxidant capacity and E.
coli F4+ and F18+ growth inhibitory activity.The concentration of 1200 µg/mL exhibited the highest E.coli growth inhibitory activity for all the samples tested.
In addition, after in vitro digestion, Qu and Qu50%−Ch50% maintained E.coli growth inhibitory activity and a modest antioxidant capacity.Three hours pre-treatment with in vitro digested Qu50%−Ch50% counteracted the H2O2 and DSS experimentally-induced stress Duvet Insert in the intestinal IPEC-J2 cells.
Ch and Qu tannin extracts, particularly when combined, may exert E.coli F4+ and F18+ growth inhibitory activity and valuable antioxidant and cell viability modulation activities.